How long can you store cdna

Hi sitara, The short answer to your question is: you should store your cDNA for as short a time as possible. The long answer to your question, based on my experience, is as follows: 1. Hope this helps. Thanks a lot for the answer. Thanks for your answer, To.

Hi sitara, If the cDNA is already degraded my recomendation is not to use it. Hi, I have a question concerning cDNA stability during transport. Thank you very much, Vanesa. Hi Vanesa,. Good luck. In the same figure, there is another example of the stability of DNA: ladders used as molecular weight markers in gel electrophoresis, such as the bp ladder shown above, can also be left out at room temperature for months without noticeable DNA degradation the ladder in the figure had been at ambient temperature for over 90 days before this run.

While this is a great technology, thermoelectric elements are notorious for failing under heavy use and are fragile, especially prone to breakage during transportation. Peltier elements have a finite lifetime and every moment a thermocycler is left on counts towards that lifespan. We will discuss advantages of NOT using Peltier in an upcoming post. One other thing. Holds at degrees can cause air moisture to condense on the metal block that holds PCR samples.

This condensation can damage the block over the long run, and if water finds its way to the underside of the block, it may drip into the electrical components causing electrical malfunction. After all, the DNA double helix has evolved to preserve our genetic information. Paleontologists amplify ancient DNA from bones deposited 50, years ago, and forensic scientists routinely collect DNA evidence from messy crime scenes.

If we can study DNA from samples that have been out in the environment for hundreds and even thousands of years, it is to be expected that our PCR bands will survive one night at room temperature. Questions about PCR?

BioCoder is the self-described newsletter of the biological revolution. We currently recommend using the cDNA within 6 months of manufacture. The date of manufacture can be determined from the lot number. The first two numbers correspond to the year, the second two digits correspond to the month and the last four digits are a consecutive number assigned to the samples we process. So, for example, a sample with lot number would have been the first sample processed in January If after this you require further help, the ECACC scientific development team will be happy to advise you.

ECACC can provide authenticated, quality cDNA in a format to suit you that allows you to progress your research project with confidence. Culture Collections. Your Cart 0 Items.